In-vitro Anti-inflammatory Activity of Selected Dihydroxy Flavone by Tpa Induced Inflammation in Polymorphonuclear Leucocytes

The present study involves the investigation of antiinflammatory activity for 2’, 4’ dihydroxy flavone by TPA [12OTechanoyl 13 – Myristate] induced inflammation in Polymorphonuclear leukocytes [PMNL]. The cell viability was assayed by Trypan blue dye exclusion assay, MTT assay, release of Cathepsin D, Nitrite and TNF-α levels. Diclofenac sodium was used as the standard drug. The selected dihydroxy flavone, 2’, 4’ dihydroxy flavone showed the significant increase in the number of viable PMNL cells and decreases the release of Cathepsin D, Nitrite and TNF-α. The present work evidenced the in-vitro anti-inflammatory effect of 2’, 4’ dihydroxy flavones. INTRODUCTION: Flavonoids are the poly phenolic compounds present almost in all parts of the flowering plants. Flavones found to possess wide pharmacological actions, especially as a potent antioxidant, 1 antinociceptive 2 , antiinflammatory 3 , antihepatotoxic 4 , antiallergic 5 , anti-platelet 6 , anti-hypertensive 7 , antiulcerogenic 8 , anti-microbial, anti-fungal, antiviral 9 , antirhinovirus 10 , antimalarial 11 and anticarcinogenic 12 . The combination of multiple pharmacological properties in a single nucleus is quite interesting. One of the earliest therapeutic applications of flavonoids is in the treatment of some inflammatory diseases. QUICK RESPONSE CODE DOI: 10.13040/IJPSR.0975-8232.6(12).5301-07 Article can be accessed online on: www.ijpsr.com DOI link: http://dx.doi.org/10.13040/IJPSR.0975-8232.6(12).5301-07 In a previous study done by the the authors, it has been proven that 2’,3’dihydroxy flavone, 2’, 4’ dihydroxy flavone, 7, 3’dihydroxy flavone and 5, 3’ dihydroxy flavone have proven to have antinociceptive 13 and anti-inflammatory action. It has been evidenced that out of four flavones, 2’, 4’dihydroxy flavone was found to have significantly potent anti –inflammatory activity 14 . This in-vitro investigation help to understand the mechanism of anti inflammatory action. Inflammation: is the biological response of vascular tissues to harmful stimuli, such as pathogens, damaged cells, or irritants 15 . It is a protective attempt by the organism to remove the injurious stimuli as well as initiate the healing process for the tissue. It is regulated to prevent over activation of the immune system and unwanted immune response. The increase of leukocytes into the site of inflammation is critical to the pathogenesis on inflammatory conditions. The ability of the selected dihydroxy flavone to inhibit tumor necrosis factor [TNF-α] which are proinflammatory cytokines, involved in fever,